anti-phospho-stat1 (py701)-pe 58d6  (Cell Signaling Technology Inc)

Journal: Molecular cell

Article Title: Transcriptional responses to IFN-γ require Mediator kinase-dependent pause release and mechanistically distinct functions of CDK8 and CDK19

doi: 10.1016/j.molcel.2019.07.034

Figure Lengend Snippet: (A) CA inhibits IFN-γ-induced STAT1 AD phosphorylation at S727. WT MEFs (± 100 nM CA, 1 h pre-treatment) were subjected to 45 min IFN-γ stimulation followed by Western blot against phosphorylated STAT1 at S727 (pS727) or Y701 (pY701) and total STAT1, CDK8, CDK19 or tubulin. STAT1 bands correspond to STAT1 α or STAT1 β isoforms.

Article Snippet: pY701 STAT1 (58D6) , Cell Signaling Technology , Cat#9167S; RRID: AB_561284.

Techniques: Phospho-proteomics, Western Blot

Journal: Molecular cell

Article Title: Transcriptional responses to IFN-γ require Mediator kinase-dependent pause release and mechanistically distinct functions of CDK8 and CDK19

doi: 10.1016/j.molcel.2019.07.034

Figure Lengend Snippet: (A) Inducible CDK8 knockout (CDK8-iKO) in CDK8fl-MEFs using 4-hydroxytamoxifen (4OHT) treatment. Cells were 4OHT-treated to activate CreERT2 or control-treated, followed by IFN-γ stimulation (45 min) and subsequent Western analysis using antibodies against phosphorylated STAT1 (pS727 or pY701 STAT1) and total STAT1, CDK8, CDK19 and tubulin.

Article Snippet: pY701 STAT1 (58D6) , Cell Signaling Technology , Cat#9167S; RRID: AB_561284.

Techniques: Knock-Out, Control, Western Blot

Journal: Molecular cell

Article Title: Transcriptional responses to IFN-γ require Mediator kinase-dependent pause release and mechanistically distinct functions of CDK8 and CDK19

doi: 10.1016/j.molcel.2019.07.034

Figure Lengend Snippet: Key Resource Table

Article Snippet: pY701 STAT1 (58D6) , Cell Signaling Technology , Cat#9167S; RRID: AB_561284.

Techniques: Virus, Recombinant, Transfection, Protease Inhibitor, Reverse Transcription, Isolation, Lysis, Gel Extraction, Plasmid Preparation, Western Blot, Expressing, Cloning, Software